garvieae (15.2%), Lc. The isolated organisms were subjected to preliminary biochemical analysis and exhibited growth at 37°C and pH 6.7 with fermented glucose, sucrose and lactose without the evolution of CO2. The species diversity was observed because eight species were also identified: P. pentosaceus (24.2%), Lc. determination of the biochemical profile of the strain and its identification. The biochemical characteristics of the most recently identified strains of A. viridans (28 strains) E. avium (28 strains) and S. uberis (9 strains, all nonhuman) were compared to the 28 strains of G. sanguinus. Gram-positive rods, not inhibited by the Vancomycin disk. inopinatus, Ped. Urease-negative. The colonies are convex, circular, and have a shiny, smooth appearance. Additionally, infection control methods may vary greatly between vancomycin resistant organisms, so informing the clinical team about the organism identification rapidly is imperative. KEY BIOCHEMICAL REACTIONS Nitrate is not reduced. lactis (15.2%), E. The genus Pediococcus comprises homofermentative coccoidal lactic acid bacteria that occur in pairs or tetrads through division in two planes. acidilactici, Ped. In addition, the similarity of the BLAST 16S rRNA sequence and biochemical tests showed that these strains in GenBank were closely linked to LAB. damnosus, Ped. urinaeequi ( Anonymous 1999).One Pediococcus species, Ped. Species often form black colonies to olive green colonies on initial isolation. Enterococci can be quickly differentiated from Pediococcus and Leuconostoc using simple biochemical tests. Morphological and biochemical identification: ... 90, 81, 96, 98, 94, 93 and 94% DNA sequence identity of known species Lactobacilli and Pediococcus registry entries . RESULTS AND DISCUSSION Bacteria isolated from the foregut, midgut and hindgut of Tilapia guineensis were identified as pediococcus pentosaceus2 and pediococcus pentosaceus1 by observing their colony morphology, The biochemical and phylogenetic analyses revealed that most of the characterized LAB belong to the genera Lactobacillus, Lactococcus, Pediococcus, Enterococcus and Leuconostoc. Table 1 Morphological and biochemical identification of isolates Isolates Form CO 2 Growth temperature NaCl 6.5% Growth pH Genus* 10°C 37°C 45°C 4.4 9.6 M101 Cocci - - + + - + - Pediococcus M102 Cocci - - + + + - - Lactococcus M103 Cocci - - + + - + - Pediococcus Identification of Pediococcus and Tetragenococcus species. Also, the organisms presented tolerance to 6.5% NaCl and 0.3% bile salt. The black pigment fades quickly upon exposure to oxygen and within a week some colonies take on a mustard yellow pigmentation. parvulus, Ped. We report the presence of Pediococcus species with probiotic properties from betel. lactis subsp. This indicates that species of LAB predominate the microbial composition of traditionally prepared dry starters in Asia, including the Eastern Himalayas. pentosaceus and Ped. The genus consists of seven species: Ped. dextrinicus, Ped. Several species of LAB in Cambodian dombea were also reported: Weissella cibaria, Lactobacillus plantarum, Lactococcus lactis, Pediococcus pentosaceus, and Enterococcus durans (Ly et al., 2018). 3.
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